Li, Jingting and Chen, Yifang and Tiwari, Manisha and Bansal, Varun and Sen, George L. and Koo, Bon-Kyoung (2021) Regulation of integrin and extracellular matrix genes by HNRNPL is necessary for epidermal renewal. PLOS Biology, 19 (9). e3001378. ISSN 1545-7885
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Abstract
Stratified epithelia such as the epidermis require coordinated regulation of stem and progenitor cell proliferation, survival, and differentiation to maintain homeostasis. Integrin-mediated anchorage of the basal layer stem cells of the epidermis to the underlying dermis through extracellular matrix (ECM) proteins is crucial for this process. It is currently unknown how the expression of these integrins and ECM genes are regulated. Here, we show that the RNA-binding protein (RBP) heterogeneous nuclear ribonucleoprotein L (HNRNPL) binds to these genes on chromatin to promote their expression. HNRNPL recruits RNA polymerase II (Pol II) to integrin/ECM genes and is required for stabilizing Pol II transcription through those genes. In the absence of HNRNPL, the basal layer of the epidermis where the stem cells reside prematurely differentiates and detaches from the underlying dermis due to diminished integrin/ECM expression. Our results demonstrate a critical role for RBPs on chromatin to maintain stem and progenitor cell fate by dictating the expression of specific classes of genes.
Item Type: | Article |
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Subjects: | Eurolib Press > Biological Science |
Depositing User: | Managing Editor |
Date Deposited: | 11 Mar 2023 07:09 |
Last Modified: | 20 Mar 2024 04:17 |
URI: | http://info.submit4journal.com/id/eprint/1034 |