Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA

García-Alegría, Alejandro Monserrat and Anduro-Corona, Iván and Pérez-Martínez, Cinthia Jhovanna and Guadalupe Corella-Madueño, María Alba and Rascón-Durán, María Lucila and Astiazaran-Garcia, Humberto and Abdel-Rehim, Mohamed (2020) Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA. International Journal of Analytical Chemistry, 2020. pp. 1-9. ISSN 1687-8760

[thumbnail of 8896738.pdf] Text
8896738.pdf - Published Version
Download (1MB)

Abstract

This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA. Microvolumes were used to analyse DNA samples. Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision value was ≤2% CV. Trueness based on bias and the percentage of recovery showed bias values lower than Z-test with a 95% confidence level and a recovery percentage within the range (% Rec = 100% ± 5%), and the stability of the samples was 60 days (2–4°C).

Item Type: Article
Subjects: Eurolib Press > Chemical Science
Depositing User: Managing Editor
Date Deposited: 13 Jan 2023 08:08
Last Modified: 11 May 2024 08:38
URI: http://info.submit4journal.com/id/eprint/1150

Actions (login required)

View Item
View Item