Zishan, Shafiul Azam and Uddin, Md. Maien and Mohammad, Mahathir and Uddin, Mohammad Eshmam and Azad, S.M. Asadul Karim and Naima, Jannatul and Ibban, Sadab Sipar (2023) In vivo and In vitro Initiatives for the Examination of Pharmacological Properties of Brassaiopsis hainla Leaves. Asian Journal of Research in Botany, 9 (1). pp. 15-28.
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Abstract
Aims: This study is intended to evaluate the methanol extract of Brassaiopsis hainla leaves (MEBH) in the parameter of helminths, thrombolytic, cytotoxic, diarrhea & pyrexia by using In vivo and in vitro tactics.
Methodology: In vivo tests, including those for anti-diarrheal and antipyretic effects, were conducted using mice that were orally dosed with MEBH, while in vitro tests were conducted using aquarium worms, human blood, and brine shrimp for a specific purpose, such as anti-helminthic analysis, clot lysis analysis, and cytotoxicity.
Results: BRASSAIOPSIS HAINLA have showed a potential effect in certain diseases, the anthelmintic potential of MEBH was demonstrated in a dose-dependent approach on aquatic worms (Tubifex Tubifex) at different concentrations. Still, the effective one is MEBH 100 (µg/ml) = 29.749 ± 1.444 times of paralysis, 15.256 ± 6.118. The MEBH demonstrated a high level of thrombolytic activity at 48.358% of clot lysis. Compared to negative control but lower than standard drug. Following 24 hours, the LC50 was assessed in a brine shrimp lethality test. The LC50 value of the extract was 328.02 µg/mL, which is relatively toxic to brine shrimp, predominantly when associated with the LC50 value of 3.8 µg/mL for typical vincristine sulfate. In the research examining its efficacy towards diarrhea, Gastrointestinal motility, and peristalsis indexes among 200 and 400 mg/kg were identified to be significantly (P < 0.005) hindered by MEBH. The highest diminution in fever elicited by MEBH at 400 mg/kg demonstrates its promising antipyretic potential.
Conclusion: Our study confirmed that MEBH possesses anthelmintic, antipyretic, cytotoxic, thrombolytic, and anti-diarrheal effects, which makes it an intriguing source for innovative treatments. This could be an alternative remedy for common diseases that have to take synthetic medication.
Item Type: | Article |
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Subjects: | Eurolib Press > Biological Science |
Depositing User: | Managing Editor |
Date Deposited: | 24 Mar 2023 12:57 |
Last Modified: | 02 Feb 2024 04:07 |
URI: | http://info.submit4journal.com/id/eprint/1476 |