In vivo and In vitro Initiatives for the Examination of Pharmacological Properties of Brassaiopsis hainla Leaves

Aims: This study is intended to evaluate the methanol extract of Brassaiopsis hainla leaves (MEBH) in the parameter of helminths, thrombolytic, cytotoxic, diarrhea & pyrexia by using In vivo and in vitro tactics.

Methodology: In vivo tests, including those for anti-diarrheal and antipyretic effects, were conducted using mice that were orally dosed with MEBH, while in vitro tests were conducted using aquarium worms, human blood, and brine shrimp for a specific purpose, such as anti-helminthic analysis, clot lysis analysis, and cytotoxicity.

Results: BRASSAIOPSIS HAINLA have showed a potential effect in certain diseases, the anthelmintic potential of MEBH was demonstrated in a dose-dependent approach on aquatic worms (Tubifex Tubifex) at different concentrations. Still, the effective one is MEBH 100 (µg/ml) = 29.749 ± 1.444 times of paralysis, 15.256 ± 6.118. The MEBH demonstrated a high level of thrombolytic activity at 48.358% of clot lysis. Compared to negative control but lower than standard drug. Following 24 hours, the LC50 was assessed in a brine shrimp lethality test. The LC50 value of the extract was 328.02 µg/mL, which is relatively toxic to brine shrimp, predominantly when associated with the LC50 value of 3.8 µg/mL for typical vincristine sulfate. In the research examining its efficacy towards diarrhea, Gastrointestinal motility, and peristalsis indexes among 200 and 400 mg/kg were identified to be significantly (P < 0.005) hindered by MEBH. The highest diminution in fever elicited by MEBH at 400 mg/kg demonstrates its promising antipyretic potential.

Conclusion: Our study confirmed that MEBH possesses anthelmintic, antipyretic, cytotoxic, thrombolytic, and anti-diarrheal effects, which makes it an intriguing source for innovative treatments. This could be an alternative remedy for common diseases that have to take synthetic medication.