Kumar, Pradeep and Alamgir, . and Kirti, Simran and Rani, Ritu and Singh, Pusphendra and Kiran, . (2023) A Simplified, Efficient and Rapid DNA Extraction Protocol from Rice Grains and Leaf. International Journal of Plant & Soil Science, 35 (21). pp. 517-524. ISSN 2320-7035
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Abstract
DNA extraction is very complicated with different plant species because the presence of secondary metabolites that hinder with DNA isolation processes and application like DNA restriction, Gene amplification, as well as gene cloning. A simple method for preparation of rice genomic DNA was developed. The current study expressed comparatively rapid, cost-effective, less time consuming, methods for DNA extraction from seed, young leaves and old leaves of rice genotypes without using liquid nitrogen, phenol, and β–mercaptoethanol in extraction buffer. Using this method, high quality and quantity of genomic DNA was get from 0.5 g of rice seed, young leaves and 75 days old leavesand extracted total nucleic acids (genomic DNA) were amplified by employing single sequence repeats (SSR) or microsatellite markers, which produced reproducible results.This protocol resolves the problems of DNA degradation, contamination, and low yield due to binding and/or coprecipitation with starches and polysaccharides.
Item Type: | Article |
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Subjects: | Eurolib Press > Agricultural and Food Science |
Depositing User: | Managing Editor |
Date Deposited: | 26 Oct 2023 08:57 |
Last Modified: | 26 Oct 2023 08:57 |
URI: | http://info.submit4journal.com/id/eprint/2788 |