Trichiurus lepturus HYDROLYSATE PEPTIDE (THP) - CONJUGATED MAGNETIC Fe3O4 NANOPARTICLES AND THEIR ANTI-CANCER ACTIVITY TOWARDS CW-2 COLORECTAL CANCER CELLS

To prepare Trichiurus lepturus hydrolysate peptide (THP) coated magnetic Fe3O4 nanoparticles and to study the anti-cancer activity towards CW-2 colorectal cancer cells. Fe3O4 magnetic nanoparticles were used as a base and small, antitumor peptides, prepared from Trichiurus lepturus, were conjugated to the Fe3O4 magnetic nanoparticles. XRD, TEM and AFM methods were applied to characterize the structure of the nanoparticles. Proliferation and Fluorescence polarization was applied to study the effects of the particles on CW-2 cell membrane fluidity in nonmagnetic and alternating magnetic fields. The result showed that the conjugated Fe3O4 magnetic nanopeptide particles were spherical with an average size of ~15 nm and it could enhance dispersion stability over an optimal pH range of 6.5-9. Membrane fluidity analysis showed that the P and η values in the experimental CW-2 cell membrane decreased significantly after 24 h, indicating that Fe3O4 magnetic nanoparticles coated with THP increased CW-2 membrane fluidity in a dose-effect dependent manner.