Can the Prediction of Intrauterine Insemination Results by Using Aniline Blue Dyeing (ABS) and Sperm Chromatin Dispersion Levels (SCD)?

This chapter aimed to perform routine seminal fluid analysis, sperm DNA fragmentation, sperm function tests at the chromatin maturation level and evaluate pregnancy in the patients passing intrauterine insemination before starting Intra Uterine Insemination (IUI) method. Intra uterine insemination together with ovarian stimulation, is a less expensive and invasive treatment in comparison with other assisted reproductive techniques. Variance in pregnancy rate achieved may be due to the small size of the study populations, variability in characteristics of subjects and ovarian stimulation and treatment protocols, including maternal age. We studied 111 couples with unexplained infertility due to both male and female factors (Unexplained infertility is a medical term used to describe a situation in which a couple is unable to conceive a child despite both partners appearing to be normal and healthy in terms of their reproductive systems) between November 2020 and February 2021 at Al-Farah Specialist Fertility Center and IVF –Baghdad, Iran. In addition, sperm Chromatin Dispersion (Halo test) and sperm maturation were performed with Aniline Blue Stain (ABS). Sperm Chromatin Dispersion groups (SCD) were compared in terms of pregnancy outcome; positive pregnancy rate was found to be above in the normal SCD groups (p=0.0005). In addition, Aniline Blue Stain (ABS) groups were compared with in the terms of pregnancy outcome; positive pregnancy rate founded to be higher in the normal ABS group (p=0.017). Infertility clinics and IVF centers in Iraq and Worldwide face a huge problem in diagnosing the cause of male infertility. The main criterion in the initial diagnosis of the cause of infertility in male couples is seminal fluid analysis. The study demonstrated that routine semen analysis in conjunction with DNA fragmentation (SCD) and sperm maturation tests (ABS) will significantly aid in the prediction of favorable outcomes for intrauterine insemination (IUI). All things considered, the research suggests that abnormalities in the fragmentation of sperm DNA may have an impact on the result of IUI.