In vitro ANTI-INFLAMMATORY ACTIVITY USING FRUIT FORMULATION MEDIATED ZINC OXIDE NANOPARTICLES

Aim: To determine the anti-inflammatory activity of zinc oxide nanoparticles prepared using Punica granatum and Elettaria cardamomum.

Background: Over the counter and prescription non-steroidal anti-inflammatory agents like Aspirin, diclofenac, and ibuprofen are the most commonly used drugs for inflammation and its associated conditions. Unfortunately, these drugs are associated with many adverse side effects. Hence search for a more potent and safe alternative has been on the rise. In the current study zinc oxide nanoparticles known for its good anti-inflammatory activity has been prepared using a green method mediated by extract from Punica granatum and Elettaria cardamomum to reduce the side effects.

Materials and Methods: Collection and preparation of Punica granatum and Elettaria cardamomum extract, synthesis of zinc oxide nanoparticles, collection of nanoparticles using centrifugation, characterization of the nanoparticles, anti-inflammatory activity of ZnO nanoparticle using ultraviolet-visible spectroscopy, and inhibition of albumin denaturation assay were performed.

Results: Punica granatum and Elettaria cardamomum mediated zinc oxide nanoparticles showed good anti-inflammatory activity which was comparable to the control Diclofenac.

Conclusion: The data from the study has shown that Punica granatum and Elettaria cardamomum mediated zinc oxide nanoparticles have shown potent anti-inflammatory property and further research can be undertaken in order to subject it for various medicinal purposes.