ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF MEDICINALLY IMPORTANT SALEP ORCHID, Satyrium nepalense D. Don (SALAM MISHRI)

Drugs derived from plant are gaining interest because of fewer adverse effect, inexpensiveness and acceptance due to long of history use. This paper reports an attempt for the evaluation of the antioxidant, antibacterial and antifungal effect of ethanol extract of in vivo plant sample of Satyrium nepalense. The antioxidant activity of in vivo tubers extracts of different dilution (0.5-10 mg/l) was determined and compared by using DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Concentrated tuber extract had maximum antioxidant activities. To determine antibacterial potential, extracts at different concentration(20, 40 mg/ml) was assessed against three gram-positive and three gram negative bacteria, where the tested sample expressed broad spectrum activity against both strains, higher being against gram-positive followed by gram-negative bacteria. Antifungal activities of extracts of different dose (20, 40 mg/ml) were too examined against Aspergillus flavus and Fusarium oxysporum, where the extract was effective against both fungi.