A Highly Selective Fluorescent Probe for Hypochlorous Acid in Living Cells Based on a Naphthalene Derivative

Sun, Jingguo and Xu, Junhong and Ma, Qiujuan and Mao, Guojiang and Zhu, Nannan and Tian, Meiju and Li, Linke and Liu, Shuzhen and Ruiz Ángel, María José (2022) A Highly Selective Fluorescent Probe for Hypochlorous Acid in Living Cells Based on a Naphthalene Derivative. International Journal of Analytical Chemistry, 2022. pp. 1-10. ISSN 1687-8760

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Abstract

Hypochlorous acid (HOCl) was crucial for maintaining the homeostasis in cells and plays vital roles in many physiological and pathological processes. In this work, a highly selective fluorescent probe for hypochlorous acid in living cells was constructed and prepared based on a naphthalene derivative. A naphthalene derivative was utilized as the fluorescent group, and N,N-dimethylthiocarbamate was applied as the selective recognition site for HOCl. Before adding HOCl, the fluorescent probe exhibited weak fluorescence. Upon adding HOCl, the fluorescent probe displayed remarkable fluorescence enhancement. The fluorescence intensity at 502 nm showed a linear response to the concentration of HOCl from 3.0 × 10−7 to 1.0 × 10−5 mol·L−1. The detection limit was estimated to be 1.5 × 10−7 mol·L−1 for HOCl. The fluorescent probe showed fast response and outstanding selectivity toward HOCl. It owned good biocompatibility and had also been successfully applied in the confocal imaging of exogenous and endogenous HOCl in living cells.

Item Type: Article
Subjects: Eurolib Press > Chemical Science
Depositing User: Managing Editor
Date Deposited: 30 Nov 2022 05:17
Last Modified: 04 May 2024 04:18
URI: http://info.submit4journal.com/id/eprint/449

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